HiMedia M089-500G Blood Agar Base with Low pH, 500 g
KES 23627
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High-quality Blood Agar Base with low pH for cultivating and identifying microorganisms.
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Product Details
- HiMedia M089-500G Blood Agar Base with Low pH, 500 g
- Dehydrated Culture
- Microbiological Culture Media
- Package Weight: 0.612 kilograms
- Blood Agar Base with Low pH (500g)
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Microbiology Labs
Ideal for laboratories conducting experiments requiring a nutrient-rich medium for bacterial growth and isolation.
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Clinical Diagnostics
Perfect for hospitals and clinics needing to culture specimens for diagnosis of bacterial infections efficiently.
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Educational Institutions
Suitable for schools and universities teaching microbiology, providing students practical hands-on experience with culturing techniques.
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Non-Scientific Use
Not suitable for hobbyists or non-professionals lacking the knowledge to safely handle microbiological materials.
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Restricted Environments
Unsuitable for environments where strict sterilization protocols cannot be maintained, risking contamination and inaccurate results.
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Isolated Research
Not recommended for research projects that require specialized media other than standard blood agar for unique bacterial species.
Product Description
HiMedia M089-500G Blood Agar Base with Low pH, 500 g
Customer Questions & Answers
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Question:
What is HiMedia M089-500G Blood Agar Base with Low pH used for?
Answer: HiMedia M089-500G Blood Agar Base with Low pH is used primarily for culturing a wide variety of microorganisms, particularly pathogenic bacteria. This formulation supports the growth of fastidious organisms due to its enriched nutritional content. It is essential in clinical microbiology laboratories for isolating and identifying pathogens such as Streptococcus and Staphylococcus species, which are common in infections. Additionally, its low pH helps in the selective isolation of anaerobes, making it invaluable for studying infections related to these microorganisms. -
Question:
How should Blood Agar Base with Low pH be prepared?
Answer: To prepare Blood Agar Base with Low pH, dissolve the powder in sterile distilled water according to the manufacturer's instructions. Usually, it requires heating to ensure complete dissolution. After achieving a homogenous solution, sterilize by autoclaving. Pour the agar into Petri dishes and allow it to solidify. This preparation provides the optimal environment for microorganism growth and ensures reliable test results when examining samples for pathogenic bacteria in clinical setups or research laboratories. -
Question:
Can I use Blood Agar Base with Low pH for indole testing?
Answer: Yes, Blood Agar Base with Low pH can be used for indole testing when specific indicators and substrates are added. While the base itself encourages the growth of certain bacteria, when supplemented with tryptophan and specific indicators, it serves as an excellent medium to test for the indole production of bacteria like Escherichia coli. This application is crucial in microbiological studies, providing clarity in differentiating species based on metabolic capabilities. -
Question:
Is Blood Agar Base with Low pH suitable for anaerobic bacteria?
Answer: Absolutely, the Blood Agar Base with Low pH is designed to facilitate the growth of anaerobic bacteria. The low pH environment helps to inhibit the growth of some aerobic bacteria, thus promoting the selective isolation of anaerobes. This is particularly useful in clinical microbiology for assessing infections like abscesses or deep tissue infections, where anaerobic bacteria play a significant role. For best results, incubate these cultures in anaerobic chambers to maintain an oxygen-free environment. -
Question:
What organisms can grow on Blood Agar Base with Low pH?
Answer: Blood Agar Base with Low pH is known for supporting a diverse range of bacterial organisms, including but not limited to Streptococcus, Staphylococcus, Escherichia coli, and Neisseria species. Its enrichment properties provide essential nutrients that fastidious organisms need for growth. In clinical microbiology, it aids in the isolation of pathogens from clinical samples like wound swabs or throat cultures, enabling proper identification and treatment of infections. -
Question:
How long can Blood Agar Base with Low pH be stored?
Answer: Blood Agar Base with Low pH can typically be stored for several months if kept in a cool, dry place, away from direct sunlight. It is essential to check for any signs of moisture or contamination before use. Proper storage minimizes the risk of degradation and maintains the medium's effectiveness. Regular examination and proper sealing can prolong shelf life, ensuring that the agar provides reliable results during microbiological testing in laboratories. -
Question:
Can I add blood to the Blood Agar Base with Low pH?
Answer: Yes, typically, the Blood Agar Base is supplemented with defibrinated blood to create a complete blood agar medium. Adding blood enhances the nutrition of the medium, allowing various fastidious organisms to thrive. It is common practice in clinical settings since the blood provides vital growth factors and aids in hemolysis identification. Make sure to mix the blood gently into the agar once it has cooled to around 45-50 degrees Celsius to avoid damaging the red blood cells. -
Question:
What precautions should I take while using Blood Agar Base?
Answer: When handling Blood Agar Base with Low pH, it is crucial to maintain sterile techniques to avoid contamination. Use aseptic methods while preparing and inoculating the medium, and always work in a clean environment. Additionally, follow safety protocols regarding handling potentially pathogenic organisms, including personal protective equipment (PPE) like gloves and lab coats. Proper waste disposal of used media and cultures is essential to maintain safety standards in the laboratory. -
Question:
Where can I buy HiMedia M089-500G Blood Agar Base with Low pH, 500 g in Kenya?
Answer: You can purchase HiMedia M089-500G Blood Agar Base with Low pH, 500 g from Ubuy. Ubuy is a reliable online retailer offering a wide range of laboratory supplies and equipment, including microbiological media. They provide an accessible platform for buyers in Kenya to find quality products for their research and clinical needs.
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