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Item #:
210032152
Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml
Item #:
210032152
KES 8965
Price Details
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What Stands Out
Optimal pH Stability
This phosphate buffer maintains a consistent pH of 7.4, ideal for various biological and chemical applications, ensuring reliable and reproducible results in laboratory experiments.
High Concentration
With a concentration of 0.5 M, this buffer offers effective stabilization for sensitive reactions, enhancing performance in protocols compared to lower concentration alternatives.
Convenient Volume
Available in a 250 ml size, it provides ample quantity for multiple experiments, minimizing waste and ensuring long-lasting usage, perfect for research labs and educational settings.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 8 ounces (226.8 grams) |
| Volume | 250 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.81 |
| Brand | eCon Lab Supply |
| Item Volume | 250 ml |
| Package Information | Bottle |
Who Should Buy?
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Biological Research
Essential for cell culture and biochemical assays, maintaining stable pH for optimal enzyme activity and molecular stability.
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Pharmaceutical Applications
Ideal for drug formulation studies, ensuring consistent pH during the development and testing phases of pharmaceutical products.
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Laboratory Use
A must-have reagent for routine experiments involving protein purification and DNA/RNA analysis, providing precise pH control.
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Food Industry
Not suitable for food preservation or processing, as the dosage and handling do not meet food safety standards.
Product Description
Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml
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Customer Questions & Answers
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Question:
What is Phosphate Buffer, PH 7.4 used for?
Answer: Phosphate Buffer, PH 7.4 is primarily used in biological and chemical applications, particularly in molecular biology and biochemistry labs. It helps maintain a stable pH environment, which is crucial for enzyme reactions, cell cultures, and various biochemical assays. By providing this stable pH, researchers can achieve more consistent and reproducible results in experiments where pH fluctuations could affect outcomes. -
Question:
Why is a pH of 7.4 important in buffers?
Answer: A pH of 7.4 is essential because it closely resembles the physiological pH of human blood. Many biological processes and enzyme activities depend on this specific pH for optimal functioning. Using a buffer with this pH helps to simulate body conditions in lab experiments, making it ideal for studying biological reactions or testing pharmaceuticals. This relevance makes it a go-to solution for researchers and laboratories working in life sciences. -
Question:
Can I use Phosphate Buffer for protein purification?
Answer: Yes, Phosphate Buffer, PH 7.4 is often used in protein purification protocols. It provides the necessary ionic composition and pH stability that help maintain protein solubility and functionality during purification processes like chromatography or precipitation. Additionally, because it minimizes protein denaturation, it is particularly beneficial when working with sensitive biomolecules, ensuring higher yield and purity of the final product. -
Question:
Is Phosphate Buffer effective for cell culture applications?
Answer: Absolutely! Phosphate Buffer, PH 7.4 is frequently employed in cell culture applications due to its ability to maintain a consistent pH level that is vital for cell viability and growth. Maintaining physiological pH helps support cellular processes, thus leading to more reliable experimental results. It’s commonly used in media formulations to stabilize the environment that cells operate in during experiments. -
Question:
How should I store the Phosphate Buffer once opened?
Answer: After opening, Phosphate Buffer, PH 7.4 should be stored in a cool, dark place, preferably at room temperature or chilled according to storage guidelines. It should be tightly sealed to prevent contamination or evaporation. For long-term use, refrigeration may be advisable, but always check the specific manufacturer’s guidelines to ensure the buffer's effectiveness remains intact for future experiments. -
Question:
What are the typical applications of this buffer in research?
Answer: Phosphate Buffer, PH 7.4 is widely utilized in various research applications, including enzyme kinetics, immunoassays, DNA/RNA isolation, and protein folding studies. Researchers often use it to maintain pH levels in reactions involving nucleic acids, enzymes, and antibodies. Its versatility makes it an essential component in many laboratory settings focused on biochemical and molecular biology studies. -
Question:
How do I prepare a phosphate buffer solution?
Answer: To prepare a Phosphate Buffer, PH 7.4 solution, start with a stock solution of monobasic and dibasic sodium phosphate. Mix the two in appropriate proportions to adjust the pH to 7.4 using a pH meter. It's crucial to ensure all reagents are of analytical grade for consistency and reliability in experiments. Following preparation, it's advisable to test the pH again before use to confirm its accuracy for research purposes. -
Question:
Is Phosphate Buffer safe to handle?
Answer: Phosphate Buffer is generally considered safe for handling in laboratory settings. However, standard safety precautions should always be taken, such as wearing gloves and protective eyewear, especially when working with concentrated solutions or fresh preparations. While it poses low toxicity, it's important to be cautious to avoid accidental ingestion or contact with eyes or skin. -
Question:
Can this buffer be used in clinical diagnostics?
Answer: Yes, Phosphate Buffer, PH 7.4 is used in various clinical diagnostic tests, particularly where biological components are analyzed. Its stable pH can enhance the reliability of test results, making it suitable for immunological assays, enzyme tests, and more. Its ability to maintain a consistent environment is vital for providing accurate diagnoses in clinical laboratories focused on patient care. -
Question:
Where can I buy Phosphate Buffer, PH 7.4 [0.5 M] - 250 Ml?
Answer: You can purchase Phosphate Buffer, PH 7.4 [0.5 M] - 250 Ml from Ubuy. They offer a wide selection of biochemical reagents, including high-quality phosphate buffers, ensuring you have access to the products you need for your laboratory or research work in Kenya.
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KES 8965
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Features & Benefits
- Comprehensive range of biological buffers for various applications.
- Ideal for protein research and molecular biology studies.
- Manufactured in ISO 9001 and 13485 certified facility in the USA.
- Adheres to strict Good Manufacturing Practices.
- Quality control tests conducted under certified clean room conditions.
- Phosphate Buffer is vital for immunochemical staining and tissue preservation.
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